We want to take a deeper look at the JenAge data from human, mouse, killifish, zebrafish and roundworm.
For each organism we have:
- at least four tissues (brain, blood, liver, skin)
- at least five timepoints (juvenile, young adult, adult, old, very old)
- repeated under different stress conditions (aging combined with stress)
For each of these data sets we have:
- 3-10 replicates
- polyA data (most lncRNAs detectable) and miRNA data (for nearly all data sets)
For each sample we will prepare beforehand:
- Genome and annotation files
- Mappings (splice-aware and multi-mapped with different tools)
- Quantifications (unique and multiple read counts)
Questions we want to tackle:
In general, we want to answer aging-related questions, that have been already answered for a specific tissue or organism, from a more general point of view. We will also have biologist and medical researchers on site to help us interpreting the data. We already have some questions in mind, which will be investigated during the festival:
- What is the susceptibility of different organs to aging: when and where (e.g. liver) does aging start?
- How does the enriched environment affect aging in comparison to a forced aging based on treadmill training of mice
- How do metabolic pathways change with aging? Which pathways are common between species?
- How does aging affect the alternate splicing machinery (differentially expressed exons, isoforms)?
- Is ear skin of mice a representative tissue for aging? In other words: is mice skin a good model for human skin?
- How do transcription factors and their associated motifs change during aging? Is there a common set of affected transcription factors among different tissues/species?
In a systematic approach we are going to have groups working on specific aging related genes. Further we will have some people working on topics spanning all of these groups.
We also strongly encourage you to take this extensive data set as a chance to raise your own questions and use your own methods.