A: Length distribution histogram of basecalled reads. The wildtype sample produced more
reads overall, but the majority of reads was shorter than 1,000 nt (median read length: 826). In the SL2 sample, the
sequenced reads were generally longer (median read length: 1342). The difference was most pronounced for very
long reads, in particular for lengths greater than 10k nt. In total, 5,543 reads were longer than 5k nt and 594 reads
were longer than 10k nt. B: Spanned regions of the reference genome for the 12 longest reads (>22k nt) from both
data sets. Five of these cover almost the complete genome and thus represent full viral RNA genomes, sequenced
directly without a cDNA step.Read More →
A: Length distribution histogram of basecalled reads. The wildtype sample produced more
reads overall, but the majority of reads was shorter than 1,000 nt (median read length: 826). In the SL2 sample, the
sequenced reads were generally longer (median read length: 1342). The difference was most pronounced for very
long reads, in particular for lengths greater than 10k nt. In total, 5,543 reads were longer than 5k nt and 594 reads
were longer than 10k nt. B: Spanned regions of the reference genome for the 12 longest reads (>22k nt) from both
data sets. Five of these cover almost the complete genome and thus represent full viral RNA genomes, sequenced
directly without a cDNA step.