FastQCFastQC Report
Wed 3 May 2017
ebov_hsa_23h_r1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_23h_r1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26470155
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT28150.023.1440141
CGCATTA67050.017.25416466-67
GCAACGA108300.016.6262538
CGTAATC41450.016.3893452
CGGTAAT20750.015.0260811
TACGAGT72550.014.95390372-73
ACAACGC77150.014.9180762-63
CGGCATT120150.014.7935642
AACGCAT85550.014.26631364-65
ACGCATT81350.013.70611166-67
TTACGAG86450.013.46383670-71
CGTGCCC111350.013.455472
CGCGATA5302.1555024E-913.1688732
TGCAACG150300.013.0946817
CGTTGTT106400.013.0335781
GATCGGA40050.013.03025794
TTCCGTC104400.012.9691839
ATCGCAT89850.012.923326542-43
GAGCGTA29850.012.7814888
CGACAAT116400.012.7534231