FastQCFastQC Report
Wed 3 May 2017
ebov_hsa_23h_r2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_23h_r2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26470155
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT28200.021.9813481
CGCGTAT4650.019.6342541
CGTAATC33200.019.1047062
CGGCATT122600.016.5004622
CGCATTA61250.015.49490166-67
GCAACGA109800.015.4792998
CCGGCAT185250.015.1484561
CGTCGGT19600.014.2195431
GGCATTA145250.014.1294513
GATCGGA36500.013.656075594
CGCGGAT19900.013.5222431
TACGAGT66900.013.45264772-73
CGTCAAT70800.013.3704711
CGGAAAT42100.013.3541881
CCGGAAT42650.013.2946431
ACAACGC72300.013.25545862-63
TTCCGTC107750.013.2029589
CGGTAAT19450.013.093931
GCGAAAT30100.012.9310051
CGACAAT114800.012.850211