FastQCFastQC Report
Wed 3 May 2017
ebov_hsa_3h_r2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_3h_r2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17203785
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA52800.020.14701594
AGATCGG75100.016.67162394
CGCGTAT4459.640644E-1116.1946531
ATCGGAA65800.012.94758994
CCCGTAT16150.012.4944511
CCGGTAT31600.012.0110341
CCGGGAT55500.011.9461111
CGGGGTT58200.011.5570081
CGGGAAT39050.011.3189771
CCGGAAT37050.011.0222721
CGGGTAT33300.09.9550911
CGCAAAT26150.09.921161
CCCGGTT36950.09.881881
ACGCAGT37950.09.7719056
CGGGGTA24950.09.6280831
TCGGAAG70500.09.54735694
CGGGCAT44650.09.2537411
CGCGTTT12201.2732926E-119.0575021
CGCGTGT16550.08.9992041
CGGATTG25800.08.9171263