FastQCFastQC Report
Wed 3 May 2017
ebov_hsa_7h_r1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_7h_r1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24688523
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65250.014.86412694
CCGGGAT77850.012.0696841
AGATCGG87150.011.61044694
CCGGAAT50550.010.9503741
CGACAAT49350.010.8396141
CGCTAAT12950.010.7759261
CGCGTTT14800.010.6861271
CCGGTAT53050.010.60971
ATCGGAA76350.010.07700594
CGGCTAT34650.010.0684161
CCGATAT29600.010.057531
CGGAAAT47750.09.93641951
TCGGGTC81300.09.7827552
CGCGTGT21050.09.7230771
CCGGGTT78600.09.70564651
CCGGCAT71950.09.6975851
CCGGTTT39900.09.5596971
GTCGGAT27150.09.2518171
CGGCAAT54950.09.227031
CGGGATC71600.09.0293692