FastQCFastQC Report
Wed 3 May 2017
ebov_hsa_7h_r2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_7h_r2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24688523
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC73550.018.3767839
GCATACG41500.018.09414914-15
CGCGTAT5350.017.9546181
CATACGA38600.016.83948114-15
GATCGGA58400.016.6013594
CGGCATA41100.016.15732412-13
AAGACGG94550.014.9404528
ATACGAG37500.014.83074716-17
CCGGGAT77100.013.3308971
CGCAAAT35400.012.8890321
CGCGTTT16200.012.1553881
AGATCGG83400.012.01994994
CCGGTTT44950.011.7533851
CGGAAAT46850.011.5842741
CCGGAAT49300.011.398271
CGGCTAT36850.011.2088471
GAAGACG128950.011.0640387
ATCGGAA70150.011.002851594
CGGGAAT60200.010.8503161
CCGGCAT73300.010.6803041