FastQCFastQC Report
Wed 3 May 2017
hsa_r2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamehsa_r2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97548052
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT1891000.19385317914908234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG191950.022.2418177
GTCTAAC192800.021.920664
TCTCCGG1015750.020.5526816
AACACGT212550.019.9522028
AGCGCTC415100.018.7401089
CTCTCCG1130400.018.5888585
TTATCCG447200.017.2564748
TAGGTCG80750.016.8786647
TCCGGCC1251400.016.7849988
AGTCTAA275150.015.8777263
TATCCGG490750.015.7641939
GTACTCG300400.015.59522828-29
ACGTCTC293000.015.502707520-21
GAGTCTA299050.015.3070322
ATCGCAC866300.015.09897768-69
CTCCGGC1426500.014.9509917
CGATCGC898500.014.76444866-67
CTAACAC295500.014.7209426
CCCCTCT1450500.014.5221722
CGACCCA830600.014.44970592-93