FastQCFastQC Report
Wed 3 May 2017
hsa_flux_r2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamehsa_flux_r2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60002178
Sequences flagged as poor quality0
Sequence length3-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT113500.046.985919
TACGAAT61400.044.7628821
AATCGTT124150.042.8033568
TACGTTA33450.040.8749851
AAATCGT139650.038.1827
CGGATTA48000.035.9708188
CAAGGTA429850.035.8192339
TCAAGGT449250.034.6720628
ATTAGTC223050.034.6712234
ATCAAGG466300.033.570317
AAAATCG166250.032.238776
ACGAATG105350.032.222852
GGAGTAT252250.031.6569359
ATTCCGT108750.031.28115
GGGCCAT503050.030.6568552
AATTCCG117800.029.111724
CGTTAAA49100.028.3703523
TTAGTCT287900.027.072255
ATCCGTA42450.026.99835494
TTGGTCG137500.026.91228382-83
Produced by FastQC (version 0.11.3)