FastQCFastQC Report
Wed 3 May 2017
mmu_r1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemmu_r1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52645238
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGAGTACAGTGACGAGTGACGATACACGCATACGACTGACGCCGTAC4029290.7653664705628266No Hit
CACGTCACGTGTACGTAGTACGTACGCATACACGCATGTACGTATATAGT4010400.7617783017715677No Hit
AGATCTCATATCGTCGCTCGTCATGCGTGTATGCGTCTGCATACGGCGCA3908380.7423995309889188No Hit
GTGCAGTGCGCACATATCACATGCTATGCGTGTATGACAGTCGTATACTG3611310.6859708754664572No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1124040.21351218888971496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG456100.067.5362154
CTCGCGT460050.067.268652
TACGGCG488300.067.1212942
ACGTACG468500.067.0834320
CGTACGC468150.067.0824121
CCGTACG460600.066.5667645
CTATGCG431200.066.33436624
TCGCGTA466500.066.1981853
ACTCGCG473750.066.1006951
CGTATAC426200.066.0104342
CGTACGT461650.065.8674746
TAGTACG474550.065.7626716
GACGATA479500.065.7130221
TCGTATA425750.065.7009241
ATGTACG476200.065.6462936
ATACGAC480300.065.3490533
TATCGTC513100.065.2257469
CGCCGTA469250.065.2189243
TCGCTCG513000.065.2124514
GTATGCG504600.065.2076729
Produced by FastQC (version 0.11.3)