FastQCFastQC Report
Wed 11 Jan 2017
ath.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameath.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16828036
Sequences flagged as poor quality0
Sequence length25-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT66800.014.5089998
GTGTGCG19500.012.6646118
TTAAACG83550.011.9348757
CGCGTAT14100.010.2445561
CGCCAAT47400.09.1422941
CGGAAAT93450.09.1247791
TGCACTA67400.09.02129426-27
CCGGCAT34200.08.992251
CCGGGAT50600.08.9324741
ATCTTAA117500.08.6847394
CGCGAAT30700.08.4996331
CTCGGAT92000.08.4075771
AATCTTA122950.08.337673
CTCGCGT28200.08.2617391
TCGGGGG35250.08.1956452
CTCGAAT107450.08.152711
GGCCGTC79600.08.074018590-91
CTTAAAC133900.08.0386296
CTGGAAT139750.07.9021831
CGGGAAT66800.07.88229231