FastQCFastQC Report
Tue 24 Jan 2017
cal3_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamecal3_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11168529
Sequences flagged as poor quality0
Sequence length25-51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT803.6307028E-927.9542661
CGGGGCG551.4270116E-424.3964461
CGCGTAG551.4270116E-424.3964461
CGCGGCA2250.021.8664441
CGGGCAG1501.6370905E-1120.8725171
CCGGGGG2950.020.4682069
GCACACG6350.019.36988312
GCCCGGG3350.018.6918057
CGCGGTT1902.5465852E-1117.6553251
CGCGTGA901.9565066E-417.3937641
CCGGCCT2005.638867E-1116.7725581
CACACGT7500.016.39983413
CCCGCCT1703.6801794E-815.7859361
CCGGGAG1157.153492E-515.5571561
CCCGGAG1301.1739316E-515.4823631
CGCTCGA3400.015.19608531
CGGGTAG2800.015.1751721
CCCGGGG4450.015.0764578
AGGGCGC1502.5065146E-615.04085236
GATCGGA8350.014.9982152