FastQCFastQC Report
Tue 24 Jan 2017
cal3_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamecal3_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11168529
Sequences flagged as poor quality0
Sequence length25-51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT655.206093E-727.4879861
CCCGGCC450.001281356824.8155441
GGTCGCC5600.021.21787344
CCGGGGG3850.020.3036279
GCCCGGG3850.020.3036277
CGCGTAT1251.9286745E-819.6539081
CGGGGCG600.006798431318.6116581
CCGGGAG1609.0403773E-1018.1463661
CGGGGGG750.001167640717.8671911
TGTAGGG6550.017.730818
CGGGGGA4450.017.56605710
ATCTCGG6700.017.53599536
GATCTCG7150.016.72538635
CGTGTAG7200.016.44029816
CCCGCCT1401.3580411E-615.95284751
CGCGGCA2252.0008883E-1115.8819471
GATCGGA7650.015.7651682
CCCGCTT2750.015.4307561
CGGGGCA2354.0017767E-1115.206121
AGCGAGC3300.015.0246634
Produced by FastQC (version 0.11.3)