FastQCFastQC Report
Tue 10 Jan 2017
ebov_hsa_3h_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_3h_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15649763
Sequences flagged as poor quality0
Sequence length25-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA46750.020.97895294
AGATCGG65450.016.6686794
CGCGTAA8850.014.5008581
ATCGGAA55300.012.95279994
CCGGGAT45650.011.546121
CGCGATA4105.3184973E-511.1787972
CCCGTAT17050.010.2149941
CCCGGTT28300.010.0411671
TCGCACG12450.09.5715652
TCGGAAG59800.09.49033494
CGGGGTT45400.09.3887131
GTCCGTT17250.09.2994631
CCGGAAT33550.09.1529531
GTCGGTT23050.08.9478871
CTCGTTT41200.08.8996261
GTGTCGT29200.08.7899046
CTCGGAT39700.08.7740891
CCGAAAT27300.08.7301081
CTCCCGT52600.08.6263771
CTCGGGT39900.08.6152391