FastQCFastQC Report
Tue 10 Jan 2017
ebov_hsa_3h_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_3h_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15649763
Sequences flagged as poor quality0
Sequence length25-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA44350.023.10163794
AGATCGG62050.017.58021794
ATCGGAA54550.014.80462894
CCCGTAT14750.012.0335261
TCGGAAG56650.011.27699694
CGCGTAT3702.725617E-411.0703451
CCGGGAT47650.010.601821
CCCGGTT29200.010.5985491
CGGGGTT48100.010.1241621
CCCCCGT31750.09.4606421
CAGATCG49200.09.43220194
CGGGGTA18550.08.8324051
TAGATCG19000.08.82999892-93
CGGGTTT36850.08.7688211
CCGGAAT30750.08.7322721
CGGGTGT38350.08.663191
GCGGGTT21100.08.6277571
CTCGCGT12203.8198777E-118.5800211
CGGGCAT38800.08.5627151
GGCGTAT14900.08.5524811