FastQCFastQC Report
Tue 10 Jan 2017
ebov_hsa_7h_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameebov_hsa_7h_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22268601
Sequences flagged as poor quality0
Sequence length25-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA48550.016.03463694
AGATCGG68950.015.52446994
CCGGGAT60550.013.350631
ATCGGAA60250.012.81991294
CGCGTAT4601.2159926E-611.8472991
CCGGAAT42950.011.4197471
CCGGCAT58900.011.41148951
CCGCAAT25550.010.8426341
CGCGGAT18750.010.6573031
AGACGGC59600.010.0582769
CGCCAAT32350.09.9673561
ATACGAG28750.09.87274716-17
CATACGA29750.09.76987314-15
GCATACG30000.09.76414814-15
CGGCATA29100.09.75400412-13
CGGCTAT31150.09.6223631
CGGGAAT47150.09.5356311
CGCGTGT20050.09.5132921
CCGGTAT44550.09.4804981
CGCGTTT12000.09.4613841