FastQCFastQC Report
Tue 10 Jan 2017
hsa_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamehsa_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96093116
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATC965950.10052228923453789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAA296000.025.4115331
TAGATCG136650.018.7116788
ACGAACG316350.017.2374428-29
TACCGCG213450.017.0850261
TGCGTCG488600.017.0151129
CGGGTGT312600.016.6423341
GCGGGTT201750.016.1366621
TACGAAT471000.016.0984332
TTTAGCG341950.015.90853810-11
GTGCGTC552450.015.7061578
CGGTATA172450.015.5023934
GTCCGAA135050.015.4846641
AACGGTA92900.015.4413436
CTATACC445150.015.40542694-95
CCGAACG113150.014.8594493
GCGGTGT563150.014.8122672
GAACGTG381000.014.59003230-31
ACTATCG219850.013.95211658-59
CGAACGG105850.013.8602084
CGCATTC566000.013.7805394-95