FastQCFastQC Report
Wed 11 Jan 2017
mmu_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemmu_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43321537
Sequences flagged as poor quality0
Sequence length25-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGAGTACAGTGACGAGTGACGATACACGCATACGACTGACGCCGTAC3310370.7641395548823672No Hit
GTGCAGTGCGCACATATCACATGCTATGCGTGTATGACAGTCGTATACTG3144040.7257452569145919No Hit
AGATCTCATATCGTCGCTCGTCATGCGTGTATGCGTCTGCATACGGCGCA2777740.6411914701918355No Hit
CACGTCACGTGTACGTAGTACGTACGCATACACGCATGTACGTATATAGT2636770.6086510734833808No Hit
CACGTCACGTGTACGTAGTACGTACGCATACACGCATGTACGTATATAGTACTCGCGTAGCGACGACATCAGTAG522040.12050357308421443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCGA379200.075.96931569
CGACTCG380500.075.6279968
ACTCGAG401850.073.8353470
ACGACTC407050.070.0828767
TTCGTCA400150.069.0421470
CGACGAC397650.068.4292661
GACGACT412550.068.1996366
GCGACGA401350.067.30459660
CGACATC411150.067.2274964
CGCGTAG393050.067.011354
CTCGCGT392900.066.92425552
TGACGAC414700.066.836165
ACGACAT410750.066.83111663
CCGTACG406000.066.5245945
CGTATAC377300.066.0496642
ATCGTGT418600.065.8746958
ACTCGCG399650.065.8380251
CGTACGT410600.065.8207246
TCGTGTA420650.065.7669959
CGTAGCG407050.065.11600556
Produced by FastQC (version 0.11.3)