FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_asp_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_asp_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21033117
Sequences flagged as poor quality0
Sequence length25-363
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC265000.125991787142153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG29001.8925446E-518017.666290-294
GCTGGTA76502.1949762E-513660.452295-296
GGGACGA51251.4776022E-46796.9077285-289
AGCTGGT315950.06615.1265295-296
GTGTACG26951.7977573E-46462.7373280-284
TGTCGAT27600.06310.5347280-284
GTAATAG96550.05411.831290-294
GTCGATT16952.7259477E-45361.1626280-284
TAATAGC66052.4541843E-45273.9067290-294
TTGTCTA81153.7045058E-44292.5635285-289
GTACGTT14854.366465E-44264.9717280-284
GATGATT131753.9059162E-43965.9377290-294
TGCATAG46455.3402054E-43749.6394280-284
GTCTATA31155.56731E-43727.5713285-289
TAAACGC15355.8846886E-43679.988270-274
CTATCGA26305.991803E-43612.2524275-279
GGACGAT48705.8700435E-43576.4019285-289
ATGATTC104206.1076746E-43343.0088290-294
TACGTTA14907.1680336E-43339.804280-284
AATAGCT84057.417721E-43108.342290-294
Produced by FastQC (version 0.11.3)