FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_asp.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_asp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18975790
Sequences flagged as poor quality0
Sequence length25-374
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG94600.022475.162350-354
CCCCCCC95550.022251.705330-339
GGTTTTT160600.013238.794300-309
GGGGGGC93303.15497E-511394.161350-354
AAACCCC276900.07678.4053320-329
GCGTATC14401.5781164E-47030.9204280-289
AACCCCC163009.6295764E-56521.9336320-329
GGGGGCC128201.7869477E-45528.212350-354
ACCCCCC129101.812125E-45489.6733320-329
ACCCGCG23502.721815E-45322.029290-299
GTTTTTT212901.6427948E-44993.3076300-309
TATTGCC87652.7841976E-44851.455290-299
GGAATTA90152.94528E-44716.9175290-299
TTAAAAA481100.04419.3525310-319
AGGGGGG55854.0693735E-44229.883340-349
CCCCCCA185553.7432593E-43819.546330-339
TTGGGGG134206.526528E-43168.6296290-299
AATCGTA32808.1867975E-43086.7458280-289
TACACGG35059.348386E-42888.595280-289
AAGGGGG92508.681563E-42873.176340-349
Produced by FastQC (version 0.11.3)