FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_asp_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_asp_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18064825
Sequences flagged as poor quality0
Sequence length25-363
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATCTCTTCGAAAGGCTCACTCTCAAGCACGACTAAGAGCCTTCTG199640.11051311042315659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGG87100.020061.12350-351
CTTTTAA129200.013524.175320-329
CCGGGGT45306.607026E-59643.065330-339
GGACCAC114307.0107715E-57643.5845300-309
CGGGGTT56351.7038571E-46201.68330-339
TTTAAAA294450.05934.194340-349
CCCCCCA179401.7271031E-44869.909300-309
GACCACT92702.7666445E-44712.3066300-309
TAAAAAC132102.8091721E-44409.0923340-349
GGTTTTC216402.5129732E-44037.2537310-319
CGTCCCA36754.7826776E-43962.1843280-289
GGGGCCC251353.3902458E-43475.877310-319
CCCGGGG280954.235761E-43109.6697350-351
GTTTTCT141806.473364E-43080.6125310-319
AAAAACC146006.8624935E-42991.9922340-349
GACTAAA49958.8349794E-42915.1208280-289
CCCCCGG60058.706274E-42909.7808330-339
GAAGTAA94009.95567E-42655.5066290-299
AGAGTTG97250.00106559292566.7625290-299
TTAAAAC170859.397189E-42556.809320-329
Produced by FastQC (version 0.11.3)