FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20037397
Sequences flagged as poor quality0
Sequence length25-368
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG278160.13882042662527475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTA35801.444205E-519446.096295-297
CCACGAC68305.2565374E-510192.829295-297
ATGCGTA15151.637901E-46892.7744280-284
GCTTATC66500.06281.2354295-297
CGAAGGA57601.8691788E-46043.1436290-294
AGCTTAT89000.05866.6025295-297
GAATGCT131751.9559186E-45284.0244295-297
TGCGTAA11303.3713388E-44863.788280-284
GCGTAAT8453.8374143E-44577.0557280-284
ACTTTAG57004.3928207E-44071.1704285-289
TATCGTT14754.8616098E-44045.541275-279
GTTGGGA97855.393929E-43557.3337290-294
CGTAATA8806.430154E-43542.2502280-284
AGCTCCG82659.2353765E-42807.704285-289
GCTCCGA54600.00134340562390.6943285-289
CATTTTA153100.00132039322273.58290-294
GACGAAG58450.00153951312233.2236285-289
TGCATCC157800.00140269852205.8625290-294
CTTAACG18200.0017799092125.0615280-284
ATCCCTT122500.00202862781894.3408285-289
Produced by FastQC (version 0.11.3)