FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_can_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_can_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16777446
Sequences flagged as poor quality0
Sequence length25-373
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG809410.4824393414826071No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA454730.2710364855294423No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG199720.11904076460743786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGC52450.032925.56295-298
GTAAGCA49651.3542573E-517391.193295-298
GGGTACT32600.013243.448290-294
TTCGTAC34353.88916E-512568.745285-289
TCGTCCT39255.0778523E-510999.653290-294
TCGTACT19806.0301918E-510902.435285-289
CGTACTG15458.6544125E-59314.701285-289
TAAGCAT70108.0985665E-58211.819295-298
TATTCGT10801.6210359E-46952.277280-284
GTGGGCG42351.4778567E-46796.3223280-284
GATAAAG72501.7324694E-45954.985290-294
TTATACA52102.2366314E-45524.458280-284
GCGGAGT24702.614E-45378.2173275-279
GTGAGTG58402.8102205E-44928.498280-284
CGTCCTC54453.420047E-44530.882290-294
CGTTAGC10804.5040197E-44207.957265-269
GGTACTG59854.131998E-44122.08290-294
AAGCATG114504.321022E-43770.6235295-298
AAACCGG23305.665755E-43705.8916270-274
CATTTGA120154.757964E-43593.3118285-289
Produced by FastQC (version 0.11.3)