FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_can.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_can.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19019920
Sequences flagged as poor quality0
Sequence length25-365
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG122300.015662.768290-294
TATTAGG74550.012847.461290-294
TTATATT156700.012224.3545315-317
TTCCATT163300.011730.29305-309
TCATTTT169250.011317.91300-304
GTCGGTC42454.827517E-511281.251285-289
TCCATTA94053.9495502E-510183.713305-309
CGTGATT26956.809991E-510154.022280-284
ATCTAAG47255.9809565E-510135.219285-289
AGGTGTC200600.09549.135295-299
CTAAGGT29258.021933E-59355.587280-284
ATTAGGT71956.93429E-58874.4795290-294
CTTAACG9801.1834739E-48144.372270-274
TTATTTT262800.07289.0273310-314
TCGGTCA40051.5039177E-46832.732285-289
TTAGGTG71251.3599705E-46721.2505290-294
AGGTTAG41350.06617.9185280-284
CATTTTC161051.15811454E-45947.086300-304
ATATTAG108451.5754116E-45887.679315-317
TATTTTA168801.2722574E-45674.041310-314
Produced by FastQC (version 0.11.3)