FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_can_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_can_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17974044
Sequences flagged as poor quality0
Sequence length25-368
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA438020.24369585386571882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT24800.073151.48290-292
GCCGGTG40958.347919E-622150.875290-292
CCGGTGA48803.5565055E-512391.78290-292
CCTCGTA20951.988107E-46185.3276275-279
GGATGAG149401.11114714E-46071.4746285-289
TCGTTGT19502.5741383E-45472.569270-274
CTCGTAC12352.6647848E-45440.566275-279
ATTGAAG179251.5995165E-45060.4087285-289
GACGAAT16404.698939E-44097.011275-279
GCTCGAG55354.269831E-44097.011280-284
TCGTACA12355.336895E-43865.6648275-279
TTGAAGC156553.65993E-43862.7844285-289
CGAAACG29956.071993E-43563.108270-274
AAGCCGG89355.9606513E-43383.9893285-289
GAAGCCG110056.028341E-43296.9678285-289
TACAAAC71207.0651306E-43184.966280-284
AGGCTAG35258.4109127E-43027.379270-274
TGAGCGC45059.192108E-42876.4177275-279
CTCGAGA45209.2534156E-42866.872280-284
CGCTCGA12250.00119197712598.1477275-279
Produced by FastQC (version 0.11.3)