FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_ctr.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_ctr.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17928706
Sequences flagged as poor quality0
Sequence length25-363
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACC48900.036145.01295-299
CGACGAA15352.59495E-516449.428275-279
TCGACCC71002.6437612E-512447.119295-299
CATGCGG46856.906541E-59431.649285-289
CGGGCAG253000.06986.1304305-306
GTTACTT63651.2747725E-46942.2275285-289
TCCCATC130258.897355E-56784.9946290-294
CCCGCCG265750.06650.9536300-304
TTCCCAT271500.06510.0957290-294
ATGCGGA40002.349331E-45523.4097285-289
CCATCGA80752.0517071E-45472.1084290-294
CCCATCG108601.855533E-45425.0796290-294
GCGTTAC17503.0512907E-45049.9746280-284
AAAGCCG49503.5977032E-44463.361285-289
CGAAGTA18305.268173E-43863.3682275-279
CCGCCGG246100.03591.0017300-304
CATCGAC111456.513448E-43171.8096290-294
ACGAAGT29400.03164.144275-279
GTCGATC11259.1446226E-42964.3455260-264
GCGGAAA48150.02823.6936285-289
Produced by FastQC (version 0.11.3)