FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_eco_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_eco_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17344978
Sequences flagged as poor quality0
Sequence length25-363
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCC53503.951027E-511756.83290-294
ATCGGGA23055.133728E-511694.895285-289
CGCGAAT7356.288701E-511162.207275-279
GTCGATC10552.3812245E-45769.672285-289
GGAACGA47902.216884E-45627.71285-289
GATAAAA120151.9926902E-45235.043290-294
CCCGTAC15702.8691857E-45225.6196275-279
GCAATCG14753.0029123E-45117.21280-284
GAGACTT126152.1966749E-44986.0513290-294
TCGCCCT75153.8975567E-44184.9290-294
TCGGGAT33854.797178E-43981.792285-289
TGTCGAT18803.8170037E-73860.4158285-289
CGGGATA25805.818502E-43656.921285-289
CAATCGG16856.478781E-43499.576280-284
TCATGTG80306.229859E-43357.0027285-289
AATCGGG14807.4670144E-43269.181280-284
CTGTCGA29757.7363267E-43171.38285-289
GCGATGG76359.6544594E-42746.0835280-284
AAACGCG7250.0010796512739.7043270-274
TAGAGCG11150.00122675242564.168270-274
Produced by FastQC (version 0.11.3)