FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_eco.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_eco.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18225383
Sequences flagged as poor quality0
Sequence length25-357
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG1141390.6262639309143736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATAC60250.032552.496310
AACAGAG140150.013994.205305-309
GCAATTC71002.1471082E-513811.887295-299
GTTATCC48202.9685572E-513563.541285-289
TAACCGA10509.860651E-58894.73275-279
CTTAACG10801.0432148E-48647.654280-284
GAAAAAC164451.15187395E-45963.1743300-304
ATTCTGC172051.2608011E-45699.7617290-294
AAGCTAA173900.05639.126290-294
ACAGAGA188551.5142244E-45200.976305-309
CAATTCC102902.7057572E-44765.034295-299
AGAAAAA411750.04763.298300-304
AAAAGAG238802.428879E-44106.5493295-299
CCTTAAC44954.7325977E-43966.6052280-284
AGCTAAA147905.589605E-43315.2266290-294
CTCGTAA14000.02918.5833265-269
TATCGGG14550.00101688882808.2588265-269
TTAACTA63802.1016604E-82794.6538280-284
GCTAAAA125250.00100209752609.831290-294
CTTTACT71000.0011806532511.2522280-284
Produced by FastQC (version 0.11.3)