FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_eco_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_eco_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16342360
Sequences flagged as poor quality0
Sequence length25-336
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA342430.20953522012732553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAA71658.551961E-57991.171285-288
CGCTAAT11652.4491324E-45670.856270-274
CGAACAA17502.9076776E-45166.022280-284
TGGGCGG52153.171087E-44705.389280-284
ATCGAAC52803.2506246E-44647.463280-284
CATATCG9553.9497024E-44496.603275-279
TCGAACA29604.4267921E-44145.034280-284
CTAAAAG147453.6216804E-43883.1294285-288
CTCTTAC73256.2560046E-43349.9797280-284
ATTGACT75356.6198246E-43256.6165280-284
CTAATGG53157.057837E-43231.801285-288
ACAACAT177455.2452664E-43226.6406285-288
TAAAAGG90356.7984645E-43168.6077285-288
CCTAATG77609.361107E-42766.9172285-288
TCCTAAT106209.392752E-42695.7034285-288
CTCGGTA9300.00115957972638.5596265-269
ACTCGGT18200.00115851922621.6455265-269
ATATCGA15150.00168952762180.3787275-279
CGTAATA8250.00175919072146.457255-259
CAGTCGA31750.0018187232080.8022270-274
Produced by FastQC (version 0.11.3)