FastQCFastQC Report
Mon 8 Feb 2016
mono6_n2_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20276337
Sequences flagged as poor quality0
Sequence length25-339
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA524090.25847370755378546No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA419170.2067286610988957No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG356880.17600812217709738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCCA243150.09597.852305
ATTAAGT130305.1075447E-58955.171300-304
CGTTGAT76301.050763E-47646.5186295-299
TTAAGTC109852.1779476E-45311.1465300-304
ATTTAAA224301.5134981E-45202.2236300-304
TTTAAAC118552.5365787E-44921.378300-304
CCGTCAA84053.1874864E-44627.637290-294
CGGTCTA14104.2039147E-44355.576280-284
GCTTGCG92403.8522342E-44209.4473290-294
TAAACTC98004.3332935E-43968.9077300-304
TTAAACT122304.4991527E-43816.382300-304
AACGATC72155.6368415E-43593.9287285-289
ACGGGCT108405.301751E-43588.127290-294
CGTCAAG56656.370842E-43432.9473290-294
TCAAGTA129257.5372256E-43009.3071290-294
CGAGCGA23609.397591E-42908.4216275-279
CAGTGAT232259.734831E-42512.075295-299
TAAAACT232809.78099E-42506.14295-299
ACGGTCT47650.00129746692448.8118280-284
CGGGCTC79550.00125617152444.7073290-294
Produced by FastQC (version 0.11.3)