FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_asp_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_asp_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15956658
Sequences flagged as poor quality0
Sequence length25-369
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT163750.010675.421310-314
CGTATTC13450.09997.714285-289
CCCCGGG100055.366875E-58736.133300-304
TTTTCCC138501.0284587E-46310.831295-299
GGAAAAT148151.1767673E-45899.7646305-309
ATCCCAT76551.884966E-45709.0146290-294
GAGATAT39050.05595.7114290-294
AGAGATA81250.05378.77290-294
CGAATAA11353.2112745E-44968.311275-279
CCCGGGA97403.051576E-44486.91300-304
TTTTTTG134052.8901472E-44346.8857310-314
TTTTTGG107103.689623E-44080.5327310-314
TGTTCGG33304.636708E-44038.1155285-289
TAACTCG10105.173101E-43933.6187285-289
CTCGCGG19504.328541E-43897.6594280-284
GAAAATT150103.6236405E-43882.079305-309
CCTATCG8755.6556007E-43769.488270-274
GTCGTGT22755.6814315E-83340.8508280-284
TTTTTTT268603.8681147E-43254.0957310-314
CCGCCGG295250.02960.3728300-304
Produced by FastQC (version 0.11.3)