FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_asp.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_asp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16997078
Sequences flagged as poor quality0
Sequence length25-362
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACGAGGTGGC263630.15510313007918186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG21350.087911.45325
GATCGAT24802.8604645E-637840.918310-314
TCGATCG10554.802132E-1032346.568305-309
TACGTAC16606.348273E-1028266.71320-324
GTCGATC11451.2804265E-523417.463290-294
CTACGTA25451.4297257E-921071.115320-324
CGATGTA22401.5588739E-920947.65310-314
GTACGTC28802.3145181E-516292.618295-299
TACGTCT17203.852408E-513640.331295-299
GCATCGA56204.40675E-511132.322305-309
ATCGATC19456.3336476E-510722.134305-309
ATGTACA97354.4076303E-59640.008315-319
CGATCGA30650.09421.054305-309
AGCTACG56850.08253.78320-324
CATCGAT42101.2364106E-47430.3623305-309
ATCGATG65151.184386E-47202.262310-314
CGTAAGG39951.5586738E-46711.638285-289
TCGATCC21951.7476708E-46577.57290-294
GATGTAC57555.6668796E-86522.7095310-314
GTACTCA41351.6698276E-46484.4004285-289
Produced by FastQC (version 0.11.3)