FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_asp_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_asp_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15134188
Sequences flagged as poor quality0
Sequence length25-365
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACAA104600.015693.398300-303
TCACAAT61502.2997088E-513345.768300-303
CGTATAA20307.01538E-510107.94285-289
AATAAGA81954.083387E-510015.433295-299
GGTCCGG26001.849461E-46313.5747290-294
TACTTGC66901.6326853E-46134.2656290-294
ATTACAT134251.09585024E-46113.7036295-299
CACAATG89601.4643473E-46106.8804300-303
GTATAAC20052.2240795E-45847.9854285-289
GGGTCCG44002.4717723E-45329.641290-294
ACTAATT44902.5739195E-45222.8105290-294
AACTAAT90052.9580772E-44557.272290-294
ATAAGAA90703.0009338E-44524.613295-299
TTACATC97703.4819983E-44200.4336295-299
CTAATTA44605.441792E-43680.559290-294
ACAATGA112104.5840102E-43660.8594300-303
CGCCACG36350.03473.7686280-284
AAGCAAT89500.00102260772620.1587290-294
TAATTAC53850.02540.281290-294
CGCCAGT50900.00122844022480.776280-284
Produced by FastQC (version 0.11.3)