FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17419894
Sequences flagged as poor quality0
Sequence length25-357
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA22050.060051.7290-294
AATCTAC45550.029070.031295
GCCTAAT35301.1643984E-518755.523290-294
ACCTACG69204.4747052E-59567.484285-289
TAATCTA29408.0765814E-59007.754290-294
CCTAATC54608.3569306E-58083.8813290-294
CAACCGT15701.7964219E-46487.7017270-274
CTAATCT53401.5986839E-46199.1567290-294
ACGCCTA31552.6041595E-45246.1963285-289
TTCTAGT41954.603828E-43945.5898275-279
TACGCCT48554.624842E-43896.2478285-289
GCATGTA38454.972657E-43826.4412280-284
AGCATGT77155.561263E-43432.6377280-284
CCGGAGT50856.7643396E-43255.0146275-279
GACATAG33050.03081.9038270-274
AGATCGT35909.3918113E-42837.24280-284
AGATATT58859.0599526E-42812.5317275-279
CCTACGC123858.598869E-42672.87285-289
TAGCGTA64100.02582.176275-279
GATATTA32300.00116960612562.1902275-279
Produced by FastQC (version 0.11.3)