FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_can_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_can_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14210821
Sequences flagged as poor quality0
Sequence length25-327
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATG52651.9466184E-514505.729290-294
CAATACA174550.08750.806300-302
TCTATGG56451.3425852E-46764.629290-294
ATACTAT60703.8806704E-44193.9956280-284
AATAACA429500.03556.352295-299
CGAAAAA225400.03388.317290-294
AATACAG231553.7650755E-43298.3225300-302
CTATGGA89608.455191E-42841.2449290-294
ATACAGG180200.02825.4778300-302
ATTCCAT185457.2447583E-42745.4897285-289
TTCCATC94159.335652E-42703.9355285-289
ATAACAA289951.1119799E-52633.9941295-299
CATAATC98900.00103013232574.07280-284
TATTGAC35250.0011864272548.9417275-279
AGTGGAG200708.485201E-42536.876285-289
GTGGAGT100700.00106796732528.0588285-289
TAACAAT228250.00109744722230.6729295-299
AGTGACG228950.02223.8528285-289
ACTAACG13000.00169778342175.8594260-264
CGATTAA30050.00174967282117.9329270-274
Produced by FastQC (version 0.11.3)