FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_can.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_can.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133515
Sequences flagged as poor quality0
Sequence length25-330
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA478400.2965255866437041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCG11302.4125402E-647578.523285-289
GACTCGA16302.5098823E-516491.943285-289
ACTCGAC14854.9996004E-512068.178285-289
GCGTTCG10059.731802E-58916.042270-274
CGCGGAA14601.04705636E-48497.956275-279
TAGTTGG194000.08313.9795290-291
CTCGACG21701.4356556E-76193.9785285-289
CGTTCGA8953.0012947E-45148.961270-274
GCTCGTA105100.05115.484285-289
AGTTGGA164151.6969943E-44912.921290-291
CGTCGAT5154.8867264E-44067.3608255-259
CCTCGCG11159.2058006E-42952.159270-274
CTCGTAG108000.02489.0618285-289
GGCACCA219103.2119147E-52453.8447285-289
GCGACTC33050.00130673742440.109280-284
AAATGGG143650.0012993692245.6135280-284
ACGGGTC16050.0019073072050.8767270-274
AGCGACT46400.02044.7668280-284
ACCACCG66000.0018101392036.5051285-289
CCCAGCG159900.0016099392017.4009280-284
Produced by FastQC (version 0.11.3)