FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_can_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_can_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14005862
Sequences flagged as poor quality0
Sequence length25-327
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG448850.320472956252175No Hit
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA347720.24826747543278666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATCT68650.021811.152290-293
CAGTCGT21753.4569057E-513768.604280-284
CAATCTT59702.6045356E-512540.499290-293
AATCTTG45604.558519E-510945.436290-293
AACATAG36205.7456087E-510340.715285-289
ATCTAGC26957.9610356E-59259.961275-279
GCGTACA12301.3265996E-47608.412270-274
AGTCGTG21601.5341515E-46932.1094280-284
CGTCCGT20403.648951E-44587.4253270-274
ATCTTGA89803.5354999E-44168.529290-293
AGAGAAC92050.04066.6367285-289
AGTCCGA23356.7848305E-104007.8574270-274
CGTACAC16305.823789E-43674.4434270-274
TGGACGG82354.9551984E-43636.5164280-284
TCACGGG26206.0185866E-43571.8884270-274
AGCGGCG37106.6374557E-43363.2876285-289
GGGGAGC114305.727712E-43275.0122285-289
ACGTAAT8307.751253E-43221.4622260-264
GTCGTGG42550.0013888752346.0017280-284
GAACAAT63900.00134244672343.248285-289
Produced by FastQC (version 0.11.3)