FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_ctr.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_ctr.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20033995
Sequences flagged as poor quality0
Sequence length25-364
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC28858.920237E-624743.39290-294
CGCGCGT14850.018026.434285-289
GCGCGTA18656.833844E-510438.9285-289
TATTACG13508.854142E-59331.33280-284
ACTGATG254000.08431.262295-297
CTGATGT155858.677228E-56870.518295-297
CGCGTAC22751.6824211E-46723.8315285-289
GTACACT53701.545177E-46646.618290-294
TAATTCG9551.9384225E-46407.0024280-284
TGATGTC126151.7054779E-45658.7144295-297
GCGTACA26903.515251E-44683.01285-289
TTAAGAC51703.4371784E-44602.4937280-284
ATATTAC53903.7359103E-44414.6367280-284
TTGGCAG142004.321723E-43770.3176285-289
GGTACCG39558.548035E-43008.2039275-279
CTGGGAC241256.237214E-42958.9504290-294
AGGGCTG245606.464161E-42906.5425290-294
CTTCCCG94708.969094E-42826.743285-289
ACAAACT191107.826874E-42801.5967285-289
GACTATC95555.2925345E-52490.308280-284
Produced by FastQC (version 0.11.3)