FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_eco_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_eco_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18186574
Sequences flagged as poor quality0
Sequence length25-364
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCTACACTGTTGGCTGTGAGGAATGCACAGTGTTTCCCTGTTTATC559660.30773250640829874No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA278340.15304696750470978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACAT72600.029315.254295-298
TCACATG77002.144573E-513820.048295-298
TAAGTTG94057.706931E-77543.106290-294
CACATGA102251.1344709E-46938.182295-298
TTGAATT104401.1826804E-46795.298290-294
ACTTAGT49101.831084E-46192.2827285-289
ATTCCCG54652.2684113E-45563.423285-289
GACGTTT31802.8527298E-45148.252280-284
TGAATTC120655.264593E-43528.0354290-294
GACACTT87105.7617674E-43490.7126285-289
ACATGAC154655.190013E-43440.4905295-298
AAGTTGT153801.0855001E-72767.604290-294
AGTTGTA112702.6375346E-102697.791290-294
GTTGTAC90354.3673936E-92617.3367290-294
TTCTATA117459.012302E-52588.685285-289
AAATAAT127300.00123069072388.382285-289
TAGTTTC36650.00157859612233.4846285-289
ACGTTTT36950.00160454172215.3506280-284
TGCCGCC141500.0015205312148.7002285-289
TATCTTT78350.02089.5266280-284
Produced by FastQC (version 0.11.3)