FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_eco.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_eco.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19427019
Sequences flagged as poor quality0
Sequence length25-380
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTGT64001.4530739E-516789.438290-294
ATCCAGC234900.09148.778295-299
ACTGCAT129455.944726E-58300.6875290-294
CGCTGTC66259.341849E-58109.615290-294
CGGACCG24850.07206.7334285-289
GCCTTAC59951.9123396E-45974.5566285-289
CAATACG13302.9176418E-45212.3403275-279
GACCTTG99655.2834774E-43594.327285-289
ATAGTCG7306.277166E-43590.1238265-269
ACATTCA103255.6720944E-43469.004285-289
TCGAGCG21107.342824E-43285.5037275-279
TCCAGCC338504.064855E-43174.3694295-299
TACCCGG39107.2604944E-63053.4924280-284
TGCATCC141407.0920115E-43039.6719290-294
TAGACGC16000.00102418312798.2395270-274
GCATCGA49900.02392.6162280-284
GTTAGAC27650.00143164862355.2502280-284
AGTTAGT30050.00148919712306.9595275-279
CAGCCAT234700.00117230852289.1436295-299
CTGCATC241250.001238652226.9927290-294
Produced by FastQC (version 0.11.3)