FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_eco_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_eco_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19787599
Sequences flagged as poor quality0
Sequence length25-358
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC251230.12696335720164936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACA74252.2593347E-513464.483295-299
CATCTAA75052.308283E-513320.958300
TCCGATC19755.7544567E-511248.809280-284
GGCTAAC51256.458204E-59753.54290-294
AATCCGT17009.237469E-59047.401285-289
CATACAT56207.765961E-58894.466295-299
AATCGGC21251.4433321E-47237.9214285-289
CGTCTAT30801.3994594E-47213.1167280-284
GAATAGT33101.6162649E-46711.9023280-284
ATACATC49651.5152652E-46711.9023295-299
CCGATCT21402.5521585E-45496.085280-284
GCACTTC98402.3806778E-45079.9688290-294
CGGCTAA14953.2145708E-44953.4893285-289
AATACTC50503.7620254E-44399.2866280-284
GCTAACC57703.8198574E-44331.62290-294
GAATCCG69104.1087816E-44133.7104285-289
AAATCGG70650.04043.0205285-289
TCTGTTA77455.1617116E-43688.049285-289
TATTTTC142504.9926067E-43507.8523290-294
ATTCAAC84004.0017767E-113400.4688285-289
Produced by FastQC (version 0.11.3)