FastQCFastQC Report
Mon 8 Feb 2016
mono6_n3_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17215084
Sequences flagged as poor quality0
Sequence length25-360
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAAC90051.1140326E-47001.539295
AAACGAG46200.06823.4697285-289
GCCCCGT56602.2004546E-45569.6875285-289
ACTAGTC68200.05546.821290-294
AGCCGCG119501.9618316E-45276.055295
GTCGCTA72200.05239.5176290-294
CGCCTAG22503.5466987E-44670.286285-289
AACGAGT38653.7621096E-44448.9375280-284
GCGCCTA36954.1260946E-44265.8228285-289
CGTAGTG12605.67003E-43752.9084265-269
CCATTGG181550.03472.8096295
CTAGCAG120406.6377455E-43141.97290-294
AGCGCCT101157.027228E-43116.6018285-289
AGAGTCC122256.843283E-43094.4226290-294
ACTTAGA56007.8974076E-43070.5613280-284
GAGGTAC41609.5082744E-42841.7456275-279
GGGAACG74209.075149E-42832.3838290-294
TAGCAGC74359.111875E-42826.6694290-294
TTAAACG20050.00103256252774.6309280-284
GCCGTAG25050.00108600182696.7007270-274
Produced by FastQC (version 0.11.3)