FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_asp_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_asp_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21254905
Sequences flagged as poor quality0
Sequence length25-334
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG15204.6909063E-636190.555305-309
CCCGCAC118600.018553.0310-312
CCGCACG110900.09920.585310-312
CATTATC73301.0908428E-47504.7266305-309
CGACCTT50301.2841714E-47290.874295-299
CATACCT62151.3070133E-47080.8877300-304
CATATCA68102.3538129E-45385.1826295-299
AGGCGAA46202.599957E-45291.933290-294
ACCCGCA52252.586495E-45264.081305-309
CGCACGC147850.04960.852310-312
CACTTAA74802.8397318E-44902.8203295-299
GTTGACA98253.2662437E-44479.1567300-304
ATACCTC66655.4108276E-43668.2268300-304
CTCTCGC122555.0816004E-43591.001300-304
AAGTTCT141656.7889015E-43106.7925300-304
AGACCCG118457.120678E-43096.0823305-309
CTCCCAA166559.3852484E-42642.3127300-304
TCTCGCA96500.00113420232533.547300-304
ACTTAAG73850.00121778342482.9448295-299
GCAGACC233300.00110494742357.893305-309
Produced by FastQC (version 0.11.3)