FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_asp.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_asp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18410955
Sequences flagged as poor quality0
Sequence length25-354
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA351710.1910330018187541No Hit
GGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCTCTCCGCTTGCA259180.1407748810422925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTAG91802.8763357E-511933.289305-309
TCGATCG10858.437343E-59615.765300-304
CTAACCG40051.4897523E-97815.0596295-299
CTTAACG20104.383303E-57785.8994290-294
TAACCGT21950.07129.6846295-299
AAGTAGC87851.5803955E-46234.923305-309
TAGGCCG15352.4123793E-45709.321285-289
CCGGACC64853.0140512E-44826.417295-299
CGGACCG34503.6964013E-44536.1323295-299
CTCGATC26903.7782732E-44524.89300-304
CAATATC81508.0571815E-54480.474300-304
GCGCGGT25701.0941808E-43410.0422285-289
CGTGCAA112150.03255.9844300-304
TAACGAT21350.00115256072631.3290-294
TTTCTCG142000.00103216022571.5398300-304
TTGCCTG142500.00103944112562.5168300-304
AACCGTG46755.4205884E-92343.2642295-299
GTACCCT72000.00160970542173.5635290-294
TTCTCGA106500.00212857761870.2108300-304
ATGAATT172200.0021248391817.614295-299
Produced by FastQC (version 0.11.3)