FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_asp_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_asp_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20166494
Sequences flagged as poor quality0
Sequence length25-326
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT210050.10415791659174867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAACT178900.012568.184310-314
GCGATCG15355.955852E-511267.592285-289
AAACTTA233950.09610.806305-309
AACAAAA331450.06783.672300-304
AAAACTT172659.660229E-56511.578310-314
ACTTTAC78051.9741035E-45761.5576310-314
TGTAACC62402.6497355E-45147.546290-294
AACTTAA240503.633365E-54674.5283305-309
AAACTTT180603.1709377E-44149.9595310-314
CAAAAAC191453.5633755E-43914.7698300-304
GCACCAG195303.7081292E-43837.5967295-299
AACTTTA156554.7650927E-43590.6226310-314
AACGATC92505.822338E-43472.5066290-294
ACGAGTC53857.328987E-43211.839285-289
ACTTAAA252352.6171074E-72970.0127305-309
GCAGAAA254906.3165853E-42940.3008295-299
CTTAAAA192007.167348E-42927.6667305-309
AGCATGC113958.835453E-42818.8403290-294
ACAAAAA401455.222966E-42800.4084300-304
TTAAAAC180350.00105394242493.4272305-309
Produced by FastQC (version 0.11.3)