FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16917763
Sequences flagged as poor quality0
Sequence length25-374
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG601780.355708966959757No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC301640.17829780450287663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGA40750.051417.992340-349
CTACGTA24300.043112.824320-329
CGTTACG6804.8317524E-638516.234350-357
GATCGAT27552.8325503E-638026.918340-349
TACGTAC20850.033497.734320-329
TACGTTA14251.1367045E-524506.236350-357
TCGGGCG30551.0448981E-522861.791300-309
CGATGTA21851.7816741E-519178.793340-349
ACGTACG27951.7492086E-518741.354320-329
ACAGCTA127300.016459.412350-357
AGCTACG45002.26712E-515520.616350-357
TACGTCT24504.7040186E-512217.395320-329
GCTACGT44051.8189894E-1211891.506350-357
CTACGTT36655.0126488E-511434.014350-357
ATCGGAT23255.648318E-511264.964330-339
CGTACGT38705.5890803E-510828.337320-329
TCGATCG13056.673004E-510703.873300-309
ATCGATG65604.817862E-510646.765340-349
GTACGTC33656.3383646E-510377.826320-329
TCTGAGC236950.08842.724330-339
Produced by FastQC (version 0.11.3)