FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_can_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_can_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20690944
Sequences flagged as poor quality0
Sequence length25-384
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG821480.39702393472235964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA25852.6491762E-844498.97360-363
ACGTACG26655.3014446E-743163.164340-349
TCGATCG10803.7289283E-1035503.035350-359
ATCGATC17809.113137E-1025849.4350-359
GTACGTC22702.392602E-516891.312340-349
GTACAGC77551.8616694E-514832.99360-363
TACGTCT19507.926774E-714747.416340-349
GATCGAT21457.4360287E-913406.741350-359
CGTACGT47503.0008305E-612108.404340-349
TACGTAC20851.8189894E-1211034.037330-339
AGCATCG56101.0673193E-610252.214350-359
CTACGTA28755.456968E-128891.195330-339
CGTTATC34701.0435895E-48287.452300-309
GATGTAC71855.6873614E-78004.8604360-363
CGATCGA45004.4623448E-87303.482350-359
GAGCATC158902.423092E-57239.134350-359
GCATCGA56150.06828.723350-359
TTAACCG13101.8644356E-46504.373300-309
CATCGAT45806.9931593E-76278.9214350-359
GCGCGTT10402.4807063E-45672.0825280-289
Produced by FastQC (version 0.11.3)