FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_can.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_can.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24224699
Sequences flagged as poor quality0
Sequence length25-384
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG1663310.6866174064742765No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA1038110.42853370438163135No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA323290.1334547025744262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG23750.039312.95360-363
TCGATCG13500.031122.75340-349
TACGTCT26800.019003.035360-363
GATCGAT29400.015878.954340-349
TACGTAC23000.013531.631350-359
ATCGATC22150.010116.649330-339
CTACGTA33200.010043.917350-359
GTACGTC31150.09991.251360-363
CGATCGA62600.08949.033340-349
CGATGTA35750.08705.664340-349
GCATCGA67800.08262.677340-349
CGTACGT62550.07463.489360-363
CGTCTGA89451.8189894E-106958.692360-363
GCTACGT52400.06853.2188350-359
CATCGAT53350.05250.3228340-349
AGCATCG59050.04743.518330-339
ATCGATG85450.04682.851340-349
ATGTACA135400.04137.441350-359
GATGTAC69300.04041.915310-319
GTCTGAG131600.03869.9192360-363
Produced by FastQC (version 0.11.3)