FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_can_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_can_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24372078
Sequences flagged as poor quality0
Sequence length25-384
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC651920.26748642442388376No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA641650.2632725859485597No Hit
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA482100.19780832803833961No Hit
GCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAAC244420.10028689387913496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGC88900.032737.42370-378
ATCGATC23450.024821.803360-369
TCGATCG16400.017746.076360-369
TACGTAC27100.013424.153370-378
CTACGTA34100.012192.528370-378
GGATCGT30505.0381037E-511927.689300-309
ACGTACG28707.094059E-1111267.349370-378
TACGTCT22350.010851.439350-359
GTACGTC30700.09479.988350-359
GCTACGT58450.08298.706370-378
ATCGGAT60601.0654669E-48004.2803340-349
TGAGCAT135201.6370905E-116457.8916360-369
GATCGAT30050.06456.6973330-339
AGCATCG63850.06077.487360-369
TACAGCT262905.366019E-105535.1016370-378
GATCGTT34800.05226.933300-309
CGTACGT70400.05167.5356350-359
CGATGTA33150.05164.3267300-309
AGCTACG117159.094947E-124968.598370-378
ACAGCTA207150.04683.1704370-378
Produced by FastQC (version 0.11.3)