FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_ctr.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_ctr.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15939690
Sequences flagged as poor quality0
Sequence length25-330
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC329300.20659121977905465No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA274450.17218026197498196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCC29007.508365E-59535.019285-289
TTCGATC12651.4571878E-47286.3115285-289
CTGTTAT45801.8727018E-46037.457285-289
ATAACGC28553.2017299E-44842.654280-284
CGGGCAG390250.04251.36305-306
AGGTGAT105053.940806E-43948.3418290-294
CGGCAGC130756.1047426E-43172.2625300-304
TGCGTTA13107.9657865E-43166.2085270-274
CCGGCAG275155.1640338E-53014.8887300-304
GGTGATC69057.944956E-43003.4272290-294
GCATTTA94952.3737812E-92912.2227285-289
AACGCAT19405.272167E-52850.6758280-284
GCGTTAA7800.00103183842798.7405270-274
TCTTCGA103009.470485E-42684.6167285-289
GTTTGTC53050.00110535172606.1785280-284
TGTTATT53850.00113893722567.461285-289
TTGCTCG27050.00120176122555.5964275-279
CGAAACA17050.00134930052432.688270-274
GCGTGTT31500.00162962472194.5679275-279
TTGGAGT66300.00172636912085.336280-284
Produced by FastQC (version 0.11.3)